Valorization of the residual bagasse of Mexican oregano (Lippia graveolens HBK) by obtaining fractions enriched in pinocembrin
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DOI:
https://doi.org/10.56845/rebs.v3i1.30Keywords:
solid waste, Mexican oregano, use, flavonoids, pinocembrinAbstract
Mexican oregano (Lippia graveolens HBK) constitutes a spice of great commercial value mainly due to the presence of thymol and carvacrol, volatile fat-soluble compounds contained in its essential oil. However, little use has been made of its high content of water-soluble phenolic compounds, with proven antioxidant capacities. Among them, it stands out its high content of pinocembrin, flavanone with properties of great nutritional importance and for health due to its protective activity against cerebral ischemia. Mexico ranks second as a world producer of Mexican oregano. During the extractive process of the essential oil derived from its leaf, a large amount of solid waste rich in flavonoids is generated, which is minimally used and even becomes a source of contamination due to its poor disposal as waste. In order to value this agro-industrial waste, it was characterized by determining the content of total phenols, antioxidant activity (DHHP) and content of pinocembrin, observing a higher content of pinocembrin in the extract of oregano without essential oil (0.766 mg / ml) with respect to that corresponding to oregano without treatment (0.659 mg / ml). The results suggest that the residual bagasse from the extraction of the essential oil can be used with great potential due to its high content of polyphenols, antioxidant capacity and pinocembrin (27.93 mg / g BS). To achieve the concentration of pinocembrin, two separation methods were evaluated: chromatographic column with silica gel (hydrophilic) and separation with automated extraction in hydrophobic solid phase (Ultra C18) with pinocembrin recovery efficiencies of 56.6 and 90.1 %, respectively, being able to generate products enriched in pinocembrin with a 74.8 % purity. This work contributes to the potential development of new products and waste recovery processes in the growing natural essential oil extraction industry.
References
Cortés, C. M. del C., Flores, M H., Orozco, A. I., León, C. C., Suárez, J Á., Estarrón, E. M., y López, M. I. (2021). Identificación y cuantificación de compuestos fenólicos de extractos hidroetanólicos de orégano mexicano (Lippia graveolens HBK) y evaluación de su capacidad antioxidante. Moléculas, 26 (3), 702.
Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO). (2005). El Orégano Mexicano: Oro Vegetal. Comisión Nacional de Biodiversidad.
Corral T. L. (2011) Aprovechamiento de los residuos que se generan en la extracción del aceite esencial de orégano (Lippia graveolensHBK.s.l.). Instituto Politécnico Nacional. Durango, México.
Chung, A. S. & Ferrara, N. (2011). Developmental and pathological angiogenesis. Annu. Rev. Cell Dev. Biol., 27:563-84.
Flores, M. H.; León, C. C.; Estarrón, E. M.; Orozco, A. I. (2016). Optimización del proceso de extracción de sustancias antioxidantes a partir del orégano mexicano (Lippia graveolens H.B.K.) utilizando la metodología de superficie de respuesta (MSR). Rev. Mex. Ing. Química. 15, 773–785.
García, P. E., Castro, A. F. Gutiérrez, U. J., & García, L. S. (2012). Revisión de la producción, composición fitoquímica y propiedades nutracéuticas del orégano mexicano. Revista mexicana de ciencias agrícolas. 3(2), 339-353.
Granados, P. J., Uribe, U. N., García, L. P., Ramos, G. M., Rivero, V. R., y Pérez, R. M. (2018). Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy. Molecules. 23-852.
Hernández, F. A., Hernández, H, J., Medrano, L. J., Valenzuela, N. L., Martín, M. S., y Madinaveitia, R. L. (2008). Producción y extracción de aceite de orégano (Lippia graveolens Kunth) bajo cultivo en la Comarca Lagunera. Revista mexicana de ciencias forestales. 2(3) 2007-1132.
Koksal, O., Gunes, E.; Orkan, O. and Ozden, M. (2010). Analysis of effective factor on information sources at Turkish oregano farms. African J. Agric. Res. 5:142-149.
Liu, R., Li, J. Z., Song, J. K., Zhou, D., Huang, C., Bai, X. Y., Xie, T., Zhang, X., Li, Y. J., Wu, C. X. and Zhang, L. (2014). Pinocembrin improves cognition and protects the neurovascular unit in Alzheimer related deficits. Neurobiology of Aging 35(6): 1275 – 1285.
Mastelić J., Jerković I., Blazević I., Poljak-Blaži M., Borović S., Ivanĉić-Baće I., Smrećki V., Žarkovic N., Brcic-Kostic K., Vikic-Topic D. (2008). Comparative study on the Antioxidant and Biological Activities of Carvacrol, Thymol and Eugenol Derivatives. J. Agric. Food Chem.56:3989–3996.
Nava P. D., Juárez, G. I., Landeta, E. O., Del Moral, S. & Rosas, M. E. (2019). Potencial bioenergético a partir de residuos agroindustriales del estado de Veracruz. Renewable Energy, Biomass & Sustainability. 2683-2658
Nyotia, N., Khong, H. Y., Ahmad, S. H, Isabel, F. L., Aimi, S. S. (2016). Isolation and synthesis of pinocembrin and pinostrobin from Artocarpus odoratissimus. Malaysian Journal of Analytical Sciences. 1156 – 1161.
Osti, C.M., Torres, V. J., Villagómez, J.R., Castelán, P. I. (2010). Estudio químico de cinco plantas mexicanas de uso común en la medicina tradicional. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 9 (5): 359 - 367
Przybyłek, I., Karpiński, T. (2019). Antibacterial Properties of Propolis. Molecules. 24(11):2047.
Rolando A. (2007). Aceite esencial de orégano: tratamiento por digestión anaeróbica de los residuos generados en su obtención . Buenos Aires, Argentina. Universidad Nacional de Luján.
Villavicencio, E.; Martínez, O.; Cano, A. y Berlanga, C. (2007). Orégano, recurso con alto potencial. Ciencia y Desarrollo. 33:60-66.
Zheng, Y., Wang, K.; Wu, Y., Chen, Y.,Chen, X., Hu, C.W., Hu, F. (2018). Pinocembrin induces ER stress mediated apoptosis and suppresses autophagy in melanoma cells. Cancer Lett. 431, 31–42.
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