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Renewable Energy, Biomass & Sustainability

Open Access

Evaluation of the effect of salinity on tRNACys processing during Bacillus subtilis spore germination using RNA-seq

DOI 10.56845/rebs.v2i1.13
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Iván Arvizu-Hernández1 , Juan Caballero-Pérez2 , George H. Jones3 , Juan Campos-Guillén1
1 Laboratorio de Bacteriología molecular, Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, 76010 Querétaro, Qro., México
2 Databiology Ltd, Oxford, OX4 4GA, United Kingdom
3 Department of Biology, Emory University, Atlanta, Georgia, USA.

Abstract

In its natural habitat, the soil bacterium Bacillus subtilis is exposed to fluctuating environmental conditions, mainly osmotic stress and nutrient deficiency stress (Marles-Wright and Lewis, 2007; Schultz et al., 2007). When nutrients are depleted, this bacterium generates spores that germinate to form vegetative cells when nutrients become available again in the medium (Bremer, 2002). In this regard, the molecular mechanisms of tRNA accumulation during sporulation are a priority, as they play an essential role in translation during spore germination and its subsequent growth phase (Keijser et al., 2007). The effects of salt stress on gene expression have been previously analyzed during germination and growth. However, the processing of tRNAs under these conditions has not been extensively studied. In this study we investigated the profile of tRNACys in B. subtilis spores and growth in the absence/presence of 1.2M NaCl.

Keywords

osmotic stress,RNA sequencing,sporulation

How to Cite

Arvizu-Hernández, I., Caballero-Pérez, J., Jones, G. H., & Campos-Guillén, J. (2020). Evaluation of the effect of salinity on tRNACys processing during Bacillus subtilis spore germination using RNA-seq. Renewable Energy, Biomass & Sustainability, 2(1), 1. https://doi.org/10.56845/rebs.v2i1.13
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References

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