Itzel Díaz-González1 ,
Joaquín Estrada-García1 ,
Eduardo Hernandez-Aguilar2 ,
Alejandro Alvarado-Lassman1 ,
Juan Manuel Méndez-Contreras1
1 Tecnológico Nacional de México/Instituto Tecnológico de Orizaba
2 Universidad Veracruzana
Abstract
Brewers’ spent grains (BSGs) are the most abundant waste generated from the craft brewing process, accounting for approximately 85% of the total byproduct obtained. The need to develop beneficial alternatives for the contribution of the industrial sector and sustainable development has increased interest in the fermentation processes used to produce biomass, using probiotic microorganisms that provide health benefits for those who consume it, obtaining byproducts rich in nutrients. Therefore, this research aimed to evaluate the growth of Lactobacillus casei in Mar, Rogosa and Sharpe broth (MRS) and to evaluate the feasibility of growing L. casei in craft beer residues. To achieve this goal, a 10% v/v inoculum of probiotic bacteria was used in both media. The process consisted of monitoring the biotransformation process at 37°C and 120 rpm for 72 hours and evaluating carbohydrate consumption and cell growth. At the end of 52 h, the carbohydrate concentration in combination with BSG was completely consumed, considering that the initial value was 16.49 g/L. In the case of the MRS medium, a value of 3.42 g/L was obtained at 72 h. Regarding the pH range with the MRS broth and with BSG, the values were 6.89-5.43 and 5-4.41, respectively. Due to the acidity of the synthetic medium, the pH of the synthetic medium was greater than that of BSG. However, L. casei managed to develop in a similar way since quite similar cell growth values were obtained in both media, so it is feasible to use BSG as a culture medium for the development of probiotic species.
Díaz-González, I., Estrada-García, J., Hernandez-Aguilar, E., Alvarado-Lassman, A., & Méndez-Contreras, J. M. (2024). Evaluation of the viability of the use of Lactobacillus casei in the removal of organic contaminants in waste from the craft brewing industry. Renewable Energy, Biomass & Sustainability, 6(1), 10–16. https://doi.org/10.56845/rebs.v6i1.91
📄Angeleri, A., Ariagno, J. I., Sardi, M., Carbia, C., Palaoro, L. A., & Rocher, A. E. (2017). Comparación del recuento celular entre un método manual y un contador automatizado en líquidos de derrame. Acta Bioquímica Clínica Latinoamericana. 51(1), 37-44, http://www.redalyc.org/pdf/535/53550497007.pdf.
📄APHA, WPCF, AWWA. (2017). Standard Methods for the Examination of Water and Wastewater, 23rd ed., American Public Health Association (APHA).
📄Bevilacqua, A., Speranza, B., Santillo, A., Albenzio, M., Gallo, M., Sinigaglia, M., & Corbo, M. R. (2019). Alginate-microencapsulation of Lactobacillus casei and Bifidobacterium bifidum: Performances of encapsulated microorganisms and bead-validation in lamb rennet. LWT, 113, 108349. https://doi.org/10.1016/j.lwt.2019.108349.
📄Caltzontzin-Rabell, V., Gutiérrez-Antonio, C., García-Trejo, J. F. & Feregino-Pérez, A. A. (2022). Conversion of organic waste through a biorefinery scheme into biofuels and value-added products: overview and perspectives. Perspectivas de la ciencia y la tecnología, 5(8), 10-17. https://revistas.uaq.mx/index.php/perspectivas/article/view/669.
📄Chang, Reynaldo, & Murillo, Liliana. (2017). Determinación espectrofotométrica, de carbohidratos aprovechables en las algas Ulva sp y Chaetomorpha sp para la producción de etanol que funcione como biocombustible, por el método de la antrona. Revista de Investigación, 41(90), 053-066. https://ve.scielo.org/pdf/ri/v41n90/art05.pdf.
📄Chávez-Altamirano, C. E., López-Calvopiña, F. G., Palate-Chicaiza, X. M., & Jacome-Pilco, C. R. (2021). Potencialidad de Biocombustibles a partir de Residuos Orgánicos. Revista Scientific, 6(21), 40–57. https://doi.org/10.29394/Scientific.issn.2542-2987.2021.6.21.2.40-57.
📄Chohan, N. A., Aruwajoye, G. S., Sewsynker-Sukai, Y., & Gueguim E. B. K. (2020). Valorization of potato peel wastes for bioethanol production using simultaneous saccharification and fermentation: Process optimization and kinetic assessment. Renewable Energy, 146, 1031-1040. https://doi.org/10.1016/j.renene.2019.07.042.
📄Dávila, J. A.; Rosenberg, M. & Cardona, C. A. (2016). A biorefinery approach for the production of xylitol, ethanol and polyhydroxybutyrate from brewer’s spent grain. AIMS Agric. Food, 1, 52–66. https://doi.org/10.3934/agrfood.2016.1.52.
📄Fiallos, B. E.M. (2022). Reducción de impactos ambientales, aumento de la eficiencia productiva en la empresa FOMM Cía. Ltda., y propuesta de gestión de los residuos orgánicos mediante compostaje aerobio. Optimización del proceso productivo para la obtención de hojas secas de Moringa Oleífera en base a la utilización del manual de minimización económica del impacto Ambiental (Manual MEDIA). Tesis de licenciatura, Escuela Politécnica Nacional.
📄Głowacki, S., Salamon, A., Sojak, M., Tulej, W., Bryś, A., Hutsol, T., Salamon, M., Kukharets, S., & Janaszek-Mańkowska, M. (2022). The Use of Brewer’s Spent Grain after Beer Production for Energy Purposes. Materials, 15(10), 3703. https://doi.org/10.3390/ma15103703.
📄Guarda, E. C., Oliveira, A. C., Antunes, S., Freitas, F., Castro, P. M., Duque, A. F., & Reis, M. A. (2021). A two-stage process for conversion of brewer’s spent grain into volatile fatty acids through acidogenic fermentation. Applied Sciences, 11(7), 3222. https://doi.org/10.3390/app11073222.
📄Insuasti, G., Pilamunga, C., Gallegos N, J. M., & Pacurucu R, A. R. (2023). Evidence of the antifungal and antimicrobial activity of lactic acid bacteria in various fermented substrates for food use. Polo del Conocimiento, 8(2), 79, https://doi.org/»10.23857/pc.v8i2.
📄Kato-Kataoka, A., Nishida, K., Takada, M., Suda, K., Kawai, M., Shimizu, K. & Rokutan, K. (2016). Fermented milk containing Lactobacillus casei strain Shirota prevents the onset of physical symptoms in medical students under academic examination stress. Beneficial Microbes, 7(2), 153–156, https://doi:10.3920/bm2015.0100.
📄Lalić, A., Karlović, A., & Marić, M. (2023). Use of Brewers’ Spent Grains as a Potential Functional Ingredient for the Production of Traditional Herzegovinian Product Ćupter. Fermentation, 9(2), 123, https://doi.org/10.3390/fermentation9020123.
📄López-Legarda, X., Taramuel-Gallardo, A., Arboleda-Echavarría, C., Segura-Sánchez, F., & Betancur, L. F. R. (2017). Comparación de métodos que utilizan ácido sulfúrico para la determinación de azúcares totales. Revista Cubana de Química, 29(2), 180-198, http://scielo.sld.cu/pdf/ind/v29n2/ind02217.pdf.
📄Mæhre, H. K., Dalheim, L., Edvinsen, G. K., Elvevoll, E. O., & Jensen, I. J. (2018). Protein determination method matters. Foods, 7(1), 5. https://doi.org/10.3390/foods7010005
📄Milew, K., Manke, S., Grimm, S., Haseneder, R., Herdegen, V., and Braeuer, A. S. (2022) Application, characterization and economic assessment of brewers’ spent grain and liquor. Journal of the Institute of Brewing, 128(3), 96-108, https://doi.org/10.1002/jib.697.
📄Mirmohammadi, R., Zamindar, N., Razavi, S. H., Mirmohammadi, M., & Paidari, S. (2021). Investigation of the possibility of fermentation of red grape juice and rice flour by Lactobacillus plantarum and Lactobacillus casei. Food Science & Nutrition, 9(10), 5370-5378, https://doi.org/10.1002/fsn3.2461.
📄Orozco, R. A. U., Montenegro, K. R. G., Tinoco, W. W., Gómez, I. S., & Blandón, J. R. H. (2018). Identificación de Lactobacillus sp con potencial probiótico a partir de sustrato fermentado de yuca (Manihot esculenta). La Calera, 18(31), 89-94, https://doi.org/10.5377/calera.v18i31.7898.
📄Pasalari, H., Gholami, M., Rezaee, A., Esrafili, A., & Farzadkia, M. (2021). Perspectives on microbial community in anaerobic digestion with emphasis on environmental parameters: A systematic review. Chemosphere, 270, 128618, https://doi.org/10.1016/j.chemosphere.2020.128618.
📄Rocafuerte Peña, J. d. I., Romero Mota, D. I., & Méndez Contreras, J. M. (2022). Acción fermentativa de Bacillus Subtilis en la bioconversión de sustratos orgánicos. UPIICSA, 8(1). https://www.ruii.ipn.mx/index.php/RUII/article/view/98/94
📄Romero-Mota, D.I., Estrada-García, J., Alvarado-Lassman, A. & Méndez-Contreras J. M. (2023). Growth kinetics of Lactobacillus acidophilus During the Anaerobic Biotransformation Process of Agro-Sugarcane Waste. Waste and Biomass Valorization, 1-11, https://doi.org/10.1007/s12649-023-02100-z.
📄Rosero, G. C., Chairez, I., & Durán-Páramo, E. (2020). Carbon/nitrogen ratio and initial pH effects on the optimization of lactic acid production by Lactobacillus casei subsp casei NRRL-441. Wulfenia Journal, 27(10), 37-59.
📄Saavedra, T. M., Figueroa, G. A., Herrera-Mendez, C. H., Gantes-Alcántar, M., Mexicano-Santoyo, L., & Mexicano-Santoyo, A. (2018). Análisis químico proximal en residuos sólidos de cerveza artesanal y su aceptación en cerdas. Abanico Veterinario, 8(3).https://doi.org/10.21929/abavet2018.83.6.
📄Sánchez-Valeriano, N., Romero-Mota, D. I., Rosas-Mendoza, E. S., Hernández-Aguilar, E., & Méndez-Contreras, J. M. (2022). Determination of kinetic parameters of the anaerobic biotransformation process of corn cob (Zea Mays L.) with Lactobacillus acidophilus. Renewable energy, biomass & sustainability, 4(1), 38-43. https://doi.org/10.56845/rebs.v4i1.67
📄da Silva Barreira, D., Lapaquette, P., Novion Ducassou, J., Couté, Y., Guzzo, J., & Rieu, A. (2022). Spontaneous prophage induction contributes to the production of membrane vesicles by the gram-positive bacterium Lacticaseibacillus casei BL23. MBio, 13(5), e02375-22, https://doi.org/10.1128/mbio.02375-22.
📄Wang, Y., Wu, J., Lv, M, Shao, Z., Hungwe, M., Wang, J., Bai, X., Xie, J., Wang, Y., & Geng, W. (2021). Metabolism Characteristics of Lactic Acid Bacteria and the Expanding Applications in Food Industry. Frontiers in Bioengineering and Biotechnology, 9, 612285, https://doi.org/10.3389/fbioe.2021.612285.
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Joaquín Estrada-García1