Optimización de la digestión anaerobia en condiciones psicrófilas mediante biopelículas vegetales: evaluación del rendimiento y calidad del biogás en un biodigestor tubular ruralOptimization of anaerobic digestion under psychrophilic conditions using plant biofilms: evaluation of biogas yield and quality in a rural tubular biodigester
Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas (UNTRM)
Abstract
Este estudio evalúa el rendimiento de un biodigestor tubular operando en condiciones psicrófilas, incorporando lianas vegetales como soporte de biopelículas para mejorar la digestión anaerobia de estiércol bovino. Se construyó un sistema de biodigestión de 12 m³ en Chachapoyas, Perú, y se cargó con una mezcla estiércol:agua (1:5). Se monitorearon parámetros fisicoquímicos, producción y calidad del biogás, aplicando el modelo de Gompertz para describir su comportamiento cinético. Los resultados muestran que, a pesar de operar a temperaturas promedio de 16.95 °C, el sistema alcanzó su tiempo de retención hidráulica (TRH) en solo 15 días, generando 3 m³ de biogás con una producción diaria de 0.2–0.3 m³. La purificación redujo el H2S en 75 % y el metano purificado alcanzó 68.18 %, validando su uso energético doméstico. El modelo de Gompertz ajustó adecuadamente los datos (R² = 0.9992), proyectando una producción potencial de 3.89 m³. El uso de biopelículas vegetales demostró mejorar la retención microbiana y estabilidad del proceso, proponiendo una solución de bajo costo y replicabilidad en zonas rurales frías.
Keywords
lianas,TRH,Gompertz,Metanogénesis,bioabonos
How to Cite
Altamirano-Cubas, A., Vergara Medina, G. A., Gosgot Angeles, W., & Iliquin-Fernandez, R. E. (2025). Optimización de la digestión anaerobia en condiciones psicrófilas mediante biopelículas vegetales: evaluación del rendimiento y calidad del biogás en un biodigestor tubular ruralOptimization of anaerobic digestion under psychrophilic conditions using plant biofilms: evaluation of biogas yield and quality in a rural tubular biodigester. Renewable Energy, Biomass & Sustainability, 7(2), 28–39. https://doi.org/10.56845/rebs.v7i2.658
📄Abdurrakhman, A., Sutiarso, L., Ainuri, M., Ushada, M., & Islam, M. P. (2024). Design of a pressure control system in biogas reactor based on PID controller with Ziegler–Nichols and auto tuning PSO. Jurnal Otomasi Kontrol dan Instrumentasi, 16(2), 104–116. https://doi.org/10.5614/JOKI.2024.16.2.5
📄Abendroth, C., Latorre-Pérez, A., Porcar, M., Simeonov, C., Luschnig, O., Vilanova, C., & Pascual, J. (2020). Shedding light on biogas: Phototrophic biofilms in anaerobic digesters hold potential for improved biogas production. Systematic and Applied Microbiology, 43(1), 126024. https://doi.org/10.1016/j.syapm.2019.126024
📄Abera, G. B., Trømborg, E., Solli, L., Walter, J. M., Wahid, R., Govasmark, E., Horn, S. J., Aryal, N., & Feng, L. (2024). Biofilm application for anaerobic digestion: A systematic review and an industrial scale case. Biotechnology for Biofuels and Bioproducts, 17(1), 1–20. https://doi.org/10.1186/s13068-024-02592-4
📄Adamu, A. A., James, J. G., Olupinla, F. S., & Iyanda, P. O. (2025). Modelling biogas production from organic waste substrates using the Gompertz equation: Parameter estimation and methane composition analysis (Issue 1).
📄Alvarez, R., & Lidén, G. (2008). The effect of temperature variation on biomethanation at high altitude. Bioresource Technology, 99(15), 7278–7284. https://doi.org/10.1016/j.biortech.2007.12.055
📄Aremanda, R. B., Debretsion, S., Tesfalem, S., & Menghisteab, R. (2023). Competence of cow manure as a sustainable feedstock for bioenergy and biofertilizer production. International Journal on Food, Agriculture and Natural Resources, 4(2), 59–67. https://doi.org/10.46676/ij-fanres.v4i2.135
📄Aridi, R., & Yehya, A. (2024). Anaerobic biodigesters heating sources: Analysis and recommendations. Renewable and Sustainable Energy Reviews, 202, 114700. https://doi.org/10.1016/j.rser.2024.114700
📄Arıman, S., & Koyuncu, S. (2022). Removal of hydrogen sulfide in biogas from wastewater treatment sludge by real-scale biotrickling filtration desulfurization process. Water Practice and Technology, 17(7), 1406–1420. https://doi.org/10.2166/wpt.2022.072
📄Babaei, A., & Shayegan, J. (2019). Effects of temperature and mixing modes on the performance of municipal solid waste anaerobic slurry digester. Journal of Environmental Health Science and Engineering, 17(2), 1077–1084. https://doi.org/10.1007/s40201-019-00422-6
📄Bahrun, M. H. V., Bono, A., Othman, N., & Zaini, M. A. A. (2022). Carbon dioxide removal from biogas through pressure swing adsorption – A review. Chemical Engineering Research and Design, 183, 285–306. https://doi.org/10.1016/j.cherd.2022.05.012
📄Barrena, M. A., Maicelo, J. L., Gamarra, O. A., Oliva, M., Leiva, S., Taramona, L. A., Huanes, M. A., & Ordinola, C. M. (2019). Biogas production and applications.
📄Biogasclean. (2016). Safe injection of air or pure oxygen into biogas. Recuperado de https://www.biogasclean.com
📄Cayetano, R. D. A., Kim, G. B., Park, J., Yang, Y. H., Jeon, B. H., Jang, M., & Kim, S. H. (2022). Biofilm formation as a method of improved treatment during anaerobic digestion of organic matter for biogas recovery. Bioresource Technology, 344, 126309. https://doi.org/10.1016/j.biortech.2021.126309
📄Chavez, S. P., & Takahashi, K. (2017). Orographic rainfall hot spots in the Andes–Amazon transition according to the TRMM precipitation radar and in situ data. Journal of Geophysical Research, 122(11), 5870–5882. https://doi.org/10.1002/2016JD026282
📄Esparza-Soto, M., Alcaraz-Ibarra, S., Lucero-Chávez, M., Jiménez-Moleón, M. del C., Mier-Quiroga, M. de los A., & Fall, C. (2025). First derivative of Gompertz equation: Identification of substrate fractions in psychrophilic anaerobic digestion. Biocatalysis and Agricultural Biotechnology, 66, 103595. https://doi.org/10.1016/j.bcab.2025.103595
📄Feghhipour, S. E., Hatamipour, M. S., Amiri, H., & Nosrati, M. (2024). Continuous biogas production and ex-situ biomethanation in a trickling bed bioreactor under mesophilic and thermophilic conditions. Process Safety and Environmental Protection, 190, 1440–1449. https://doi.org/10.1016/j.psep.2024.07.095
📄Ferrer, I., Gamiz, M., Almeida, M., & Ruiz, A. (2009). Pilot project of biogas production from pig manure and urine mixture at ambient temperature in Ventanilla (Lima, Peru). Waste Management, 29(1), 168–173. https://doi.org/10.1016/j.wasman.2008.02.014
📄Ferrer, I., Garfí, M., Uggetti, E., Ferrer-Martí, L., Calderón, A., & Velo, E. (2011). Biogas production in low-cost household digesters at the Peruvian Andes. Biomass and Bioenergy, 35(5), 1668–1674. https://doi.org/10.1016/j.biombioe.2010.12.036
📄Ferrer, I., Uggetti, E., Poggio, D., Martí, J., & Velo, E. (2015). Production of biogas from organic waste in low-cost biodigesters. Recuperado de http://www.upc.edu/grecdh
📄Garfí, M., Martí-Herrero, J., Garwood, A., & Ferrer, I. (2016). Household anaerobic digesters for biogas production in Latin America: A review. Renewable and Sustainable Energy Reviews, 60, 599–614. https://doi.org/10.1016/j.rser.2016.01.071
📄Gong, W. J., Liang, H., Li, W. Z., & Wang, Z. Z. (2011). Selection and evaluation of biofilm carrier in anaerobic digestion treatment of cattle manure. Energy, 36(5), 3572–3578. https://doi.org/10.1016/j.energy.2011.03.068
📄Hadiyanto, H., Octafalahanda, F. M., Nabila, J., Jati, A. K., Christwardana, M., Kusmiyati, K., & Khoironi, A. (2023). Preliminary observation of biogas production from a mixture of cattle manure and bagasse residue in different composition variations. International Journal of Renewable Energy Development, 12(2), 390–395. https://doi.org/10.14710/ijred.2023.52446
📄Hagos, G. K., Golie, W. M., Belete, F. A., & Gidey, Y. H. (2025). Biogas upgrading produced through anaerobic co-digestion of organic biowastes: A comparative study. Biomass Conversion and Biorefinery, 1–23. https://doi.org/10.1007/s13399-025-06757-5
📄Kafle, G. K., & Chen, L. (2016). Comparison on batch anaerobic digestion of five different livestock manures and prediction of biochemical methane potential (BMP) using different statistical models. Waste Management, 48, 492–502. https://doi.org/10.1016/j.wasman.2015.10.021
📄Karne, H., & Bhatkhande, D. (2022). Effect of mixing and agitator type on biogas production from food waste in a pilot plant digester. Waste and Biomass Valorization, 13(4), 1885–1895. https://doi.org/10.1007/s12649-021-01633-5
📄Kashyap, D. R., Dadhich, K. S., & Sharma, S. K. (2003). Biomethanation under psychrophilic conditions: A review. Bioresource Technology, 87(2), 147–153. https://doi.org/10.1016/S0960-8524(02)00205-5
📄Kasinath, A., Fudala-Ksiazek, S., Szopinska, M., Bylinski, H., Artichowicz, W., Remiszewska-Skwarek, A., & Luczkiewicz, A. (2021). Biomass in biogas production: Pretreatment and codigestion. Renewable and Sustainable Energy Reviews, 150, 111509. https://doi.org/10.1016/j.rser.2021.111509
📄Kavan Kumar, V., Mahendiran, R., Subramanian, P., Karthikeyan, S., Surendrakumar, A., Kumargouda, V., Ravi, Y., Choudhary, S., Singh, R., & Verma, A. K. (2023). Optimization of biogas potential using kinetic models, response surface methodology, and instrumental evidence for biodegradation of tannery fleshings during anaerobic digestion. Open Life Sciences, 18(1). https://doi.org/10.1515/biol-2022-0721
📄Kinyua, M. N., Rowse, L. E., & Ergas, S. J. (2016). Review of small-scale tubular anaerobic digesters treating livestock waste in the developing world. Renewable and Sustainable Energy Reviews, 58, 896–910. https://doi.org/10.1016/j.rser.2015.12.324
📄Li, S, Ou, X., Wang, D., & Wang, W. (2025). Optimizing biogas production from swine manure: Biogas recirculation coupled with pH adjustment to mitigate lime inhibition. Process Safety and Environmental Protection, 198, 107234. https://doi.org/10.1016/j.psep.2025.107234
📄Lindmark, J., Thorin, E., Bel Fdhila, R., & Dahlquist, E. (2014). Effects of mixing on the result of anaerobic digestion: Review. Renewable and Sustainable Energy Reviews, 40, 1030–1047. https://doi.org/10.1016/j.rser.2014.07.182
📄Liu, Y., Wang, T., Xing, Z., Ma, Y, Nan, F., Pan, L., & Chen, J. (2022). Anaerobic co-digestion of Chinese cabbage waste and cow manure at mesophilic and thermophilic temperatures: Digestion performance, microbial community, and biogas slurry fertility. Bioresource Technology, 363, 127976. https://doi.org/10.1016/j.biortech.2022.127976
📄Lohani, S. P., & Havukainen, J. (2018). Anaerobic digestion: Factors affecting anaerobic digestion process. En Energy, environment, and sustainability (pp. 343–359). https://doi.org/10.1007/978-981-10-7413-4_18
📄Marengo, J. A., Tomasella, J., Soares, W. R., Alves, L. M., & Nobre, C. A. (2012). Extreme climatic events in the Amazon basin. Theoretical and Applied Climatology, 107(1–2), 73–85. https://doi.org/10.1007/s00704-011-0465-1
📄Marle, N. van. (1997). Characterization of changes in potato tissue during cooking in relation to texture development.
📄Martí-Herrero, J., Chipana, M., Cuevas, C., Paco, G., Serrano, V., Zymla, B., Heising, K., Sologuren, J., & Gamarra, A. (2014). Low cost tubular digesters as appropriate technology for widespread application: Results and lessons learned from Bolivia. Renewable Energy, 71, 156–165. https://doi.org/10.1016/j.renene.2014.05.036
📄Mohmed Moffit, M. A., Suja’, F., Kabir Ahmad, I., & Bhaskaran, A. N. (2025). Biogas production and reactor performance of a pilot scale anaerobic biofilm digester treating food waste. Renewable Energy, 243, 122414. https://doi.org/10.1016/j.renene.2025.122414
📄Nallamothu, R. B., Teferra, A., & Rao, B. V. A. (2013). Biogas purification, compression and bottling (Vol. 2, Issue 6).
📄Ni, J. Q. (2024). A review of household and industrial anaerobic digestion in Asia: Biogas development and safety incidents. Renewable and Sustainable Energy Reviews, 197, 114371. https://doi.org/10.1016/j.rser.2024.114371
📄Njoki, M. K., Ergas, S. J., Cunningham, J., & Wilkie, A. C. (2013). Effect of solids retention time on the denitrification potential of anaerobically digested swine waste.
📄Ofon, U. A., Ndubuisi-Nnaji, U. U., Udofia, G. E., Adegoke, A. A., Orji, E. E., Ekaette, M. I., Ukot, C. A., Offiong, N. A. O., Fapojuwo, D. P., & Shaibu, S. E. (2025). Optimization of biogas production with rice straw-derived biochar: Characterization, hormetic effects, and kinetics modelling. Cleaner Waste Systems, 11, 100288. https://doi.org/10.1016/j.clwas.2025.100288
📄Ortega-Castro, J., Herrera-Brunett, G. A., Frey E, C., Oswaldo, J., & Castro, O. (2025). Generation of biogas from solid waste from cattle at the Tunshi Experimental Station. Journal of Natural Resources Production and Sustainability, 4(1), 54–74. https://doi.org/10.61236/renpys.v4i1.1020
📄Pasalari, H., Esrafili, A., Rezaee, A., Gholami, M., & Farzadkia, M. (2021). Electrochemical oxidation pretreatment for enhanced methane potential from landfill leachate in anaerobic co-digestion process: Performance, Gompertz model, and energy assessment. Chemical Engineering Journal, 422, 130046. https://doi.org/10.1016/j.cej.2021.130046
📄Pera, L., Gandiglio, M., Marocco, P., Pumiglia, D., & Santarelli, M. (2024). Trace contaminants in biogas: Biomass sources, variability and implications for technology applications. Journal of Environmental Chemical Engineering, 12(6), 114478. https://doi.org/10.1016/j.jece.2024.114478
📄Pilarski, G., Kyncl, M., Stegenta, S., & Piechota, G. (2020). Emission of biogas from sewage sludge in psychrophilic conditions. Waste and Biomass Valorization, 11(7), 3579–3592. https://doi.org/10.1007/s12649-019-00707-9
📄Poveda, G., Espinoza, J. C., Zuluaga, M. D., Solman, S. A., Garreaud, R., & van Oevelen, P. J. (2020). High impact weather events in the Andes. Frontiers in Earth Science, 8, 162. https://doi.org/10.3389/feart.2020.00162
📄Rajendran, K., Aslanzadeh, S., & Taherzadeh, M. J. (2012). Household biogas digesters—A review. Energies, 5(8), 2911–2942. https://doi.org/10.3390/en5082911
📄Ramaiyulis, U., Mohtar Lutfi, R., Hendriani, R., & Nefri, J. (2021). Biogas installations for harvesting energy and utilization of natural fertilisers. International Journal of Scientific & Technology Research, 10(1), 1–14. https://doi.org/10.1515/agriceng-2020-0001
📄Rascón, J., Gosgot Angeles, W., Quiñones Huatangari, L., Oliva, M., & Barrena Gurbillón, M. Á. (2021). Dry and wet events in Andean populations of northern Peru: A case study of Chachapoyas, Peru. Frontiers in Environmental Science, 9, 614438. https://doi.org/10.3389/fenvs.2021.614438
📄Ravikumar, D., Hoysall, C. N., & Dasappa, S. (2020). Predicting biomethanation pattern from feedstock composition for biomass residues. En Bioresource utilization and bioprocess (pp. 75–79). https://doi.org/10.1007/978-981-15-1607-8_8
📄Riau, V., De la Rubia, M. Á., & Pérez, M. (2010). Temperature-phased anaerobic digestion (TPAD) to obtain class A biosolids: A semi-continuous study. Bioresource Technology, 101(8), 2706–2712. https://doi.org/10.1016/j.biortech.2009.11.101
📄Rivas-Solano, O., Faith-Vargas, M., & Guillén-Watson, R. (2016). Biodigesters: Chemical, physical and biological factors related to their productivity. Revista Tecnología en Marcha, 29(5), 47–53. https://doi.org/10.18845/tm.v29i5.2516
📄Rodríguez-Jiménez, L. M., Pérez-Vidal, A., & Torres-Lozada, P. (2022). Research trends and strategies for the improvement of anaerobic digestion of food waste in psychrophilic temperature conditions. Heliyon, 8(10), e11174. https://doi.org/10.1016/j.heliyon.2022.e11174
📄Shinde, S., Mangate, L., Gokhale, D., Dongardive, S., Dugge, A., Gaikwad, S., & Garware, P. (2024). Domesticating biogas – A viable alternative to LPG in India. International Research Journal on Advanced Engineering and Management (IRJAEM), 2(05), 1353–1360. https://doi.org/10.47392/irjaem.2024.0186
📄Song, Y., Qiao, W., Westerholm, M., Huang, G., Taherzadeh, M. J., & Dong, R. (2023). Microbiological and technological insights on anaerobic digestion of animal manure: A review. Fermentation, 9(5), 436. https://doi.org/10.3390/fermentation9050436
📄Swinbourn, R., Li, C., & Wang, F. (2024). A comprehensive review on biomethane production from biogas separation and its techno-economic assessments. ChemSusChem, 17(19), e202400779. https://doi.org/10.1002/cssc.202400779
📄Tian, P., Gong, B., Bi, K., Liu, Y., Ma, J., Wang, X., Ouyang, Z., & Cui, X. (2023). Anaerobic co-digestion of pig manure and rice straw: Optimization of process parameters for enhancing biogas production and system stability. International Journal of Environmental Research and Public Health, 20(1), 804. https://doi.org/10.3390/ijerph20010804
📄Tiwari, B. R., Rouissi, T., Brar, S. K., & Surampalli, R. Y. (2021). Critical insights into psychrophilic anaerobic digestion: Novel strategies for improving biogas production. Waste Management, 131, 513–526. https://doi.org/10.1016/j.wasman.2021.07.002
📄Wu, J., Zhang, H., Zhao, Y., Yuan, X., & Cui, Z. (2023). Characteristics of biogas production activity and microbial community during sub-moderate temperature anaerobic digestion of wastewater. Fermentation, 9(10). https://doi.org/10.3390/fermentation9100903
📄Xu, Z., & Chang, L. (2017). Identification and control of common weeds: Volume 3 (Vol. 3, pp. 1–944).
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Gino Alfredo Vergara Medina